SC2: Making Antibody Libraries in Phage and Yeast
Monday 12 November 2018 | 09:00 – 12:00
Room Location: Room 5B
Instructor:
Andrew R.M. Bradbury, MB BS, PhD, CSO, Specifica, Inc.
In this short course, students will learn about antibody basics, including structure, genetics and the generation of diversity, as well as the creation of naive antibody libraries in the phage and yeast display formats. This will include a description of phage and yeast display technologies, the creation of naïve libraries from natural and synthetic sources. The seminar will be fully interactive with students provided ample opportunities to discuss technology with instructors.
Antibody Background
Structure
Genes
Generation of diversity (recombination, somatic hypermutations)
Display Technologies Overview
Phage
Yeast
Combining phage and yeast display
Generation of Naïve Antibody Libraries
Natural libraries (methods, quality control)
Synthetic libraries (including strategies for generation of diversity)
Next-Generation Sequencing in Antibody Engineering
Platforms: advantages and disadvantages
Error rates and why they’re important
Naïve library diversity analysis
Selection analysis
Instructor Biography:
Andrew R.M. Bradbury, MB BS, PhD, CSO, Specifica, Inc.
Andrew Bradbury was trained in medicine at the universities of Oxford and London, and subsequently practiced medicine for five years (one full time, and four part time) in the U.K. He received his Ph.D. (Cambridge University) in the MRC Laboratory of Molecular Biology under the guidance of Dr. Cesar Milstein. After his Ph.D. he spent 10 years in Italy: three years as a post doc in the CNR Institute of neurobiology, Rome, Italy; and seven years in Trieste, where he was first visiting professor, and subsequently tenured as assistant professor at the International School for Advanced Studies (SISSA, Trieste, Italy). He was a staff scientist and group leader at Los Alamos National Lab from July 1999 to June 2017, when he left to join Specifica, a startup he founded that specializes in antibody selections and selling unique antibody libraries. He has worked in the field of phage display and antibody engineering for 25 years, and has helped organize over forty international congresses and practical courses in this field, both in Europe and the U.S. He has published over 130 peer-reviewed articles, including a number of reviews and commentaries on phage display and antibody engineering. He is one of the founding members of “The Antibody Society”, and is on the editorial board of three journals.