SC7: Protein Aggregation: Mechanism, Characterization and Consequences
Thursday 21 November, 17:30 - 20:30
Co-Instructors:
Thomas Laue, Ph.D., Professor Emeritus, Molecular, Cellular and Biomedical Sciences, University of New Hampshire and
Kevin Mattison, Principal Scientist, Malvern Panalytical Inc.
About this Course:
Protein aggregation is recognized by regulatory agencies and the biopharmaceutical industry as a key quality attribute of biotherapeutics. Various aggregates hold the potential for adversely impacting production and patients in a variety of ways. This
in-depth course reviews the origins and consequences of aggregation in biotherapeutics, and then examines strategies for predicting and quantifying aggregation in biopharmaceuticals. It benefits scientists engaged in the development, production, analytical
characterization and approval of biotherapeutics and who require a good working knowledge of protein aggregation.
What You Will Learn:
- Which phenomena are described by the term “protein aggregation”?
- How does a protein aggregate form, and what factors influence their formation?
- What are the mechanisms of protein aggregations?
- How do aggregate size, morphology and surface chemistry determine immunogenicity?
- How can protein aggregation be predicted?
- What changes may be made to the protein and solvent to minimize aggregation?
- What are the key analytical challenges in characterizing protein aggregates?
- Analytical tools for prediction and quantification of protein aggregates
Speakers Biography:
Thomas Laue, Ph.D., Professor Emeritus, Biochemistry and Molecular Biology; Director, Biomolecular Interaction Technologies Center (BITC), University of New Hampshire
Tom Laue is the Carpenter Professor of Molecular, Cellular and Biomedical Sciences, and professor of Material Sciences at the University of New Hampshire. He is the Director of both the Center to Advance Molecular Interaction Science and the Biomolecular
Interaction Technologies Center. He received his bachelor’s degree in Natural Sciences from the Johns Hopkins University in 1971 and his Ph.D. in Biophysics and Biochemistry from the University of Connecticut in 1981. His post-doctoral studies
were conducted at the University of Oklahoma. Between 1969 and 1975, he worked as a technician in the deep space program of NASA. He joined the University of New Hampshire in 1984 as an Assistant Professor, and teaches both undergraduate and graduate
courses in biochemistry and biophysics. His research focuses on the development of instrumentation and methods that use the fundamental properties of mass and charge for examining macromolecular interactions. These instruments provide unique insights
into these interactions, and have resulted in extensive collaborations with both academic and industrial labs. Tom has over 120 publications, serves on several editorial boards, and gives over one hundred lectures, seminars and workshops a year.
Kevin Mattison, Principal Scientist, Malvern Panalytical Inc.
Dr Mattison completed his doctorate from Purdue University in 1999, where he studied the effects of formulation additives on enzyme stability and activity. From there he joined Protein Solutions as the Applications Development and Technical Support Manager. In 2002 Dr. Mattison joined Malvern Instruments, where he served as Applications Manager, Product Manager, and Director of Customer Support, prior to assuming his current position as Principal Scientist in the Malvern Panalytical Biosciences group.